LSI N-MYC (Orange)

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Probe location

N-MYC

Probe description

The LSI N-MYC FISH kit is intended for the determination of N-MYC gene amplification in human tissues using fluorescence in situ hybridization (FISH).
The LSI N-MYC FISH kit contains a directly labeled fluorescent DNA probe in hybridization buffer. The fluorochrome Orange labeled N-MYC probe covers the chromosome 2p24.1 region.
The N-MYC (human homolog of v-MYC gene isolated from the avian myelocytomatosis virus, neuroblastoma derived) gene  belongs to the MYC family of transcription factors and plays a role in the regulation of cell growth and proliferation. Amplification of this gene is associated with a variety of tumors, most frequently neuroblastomas. N-MYC amplification is an important unfavorable prognostic factor of this disease.

FISH results

A normal cell hybridized with the LSI N-MYC (Orange) (1A). The cell in this image shows 2 orange signals. An abnormal cell (1B) shows more than 20 orange signals which indicate the N-MYC gene amplification.

A normal cell hybridized with the LSI N-MYC (Orange) shows 2 orange signals of the N-MYC gene. An abnormal cell hybridized with the LSI N-MYC (Orange) shows more than 20 orange signals which indicate the amplification of N-MYC gene.
A B

Figure 1. The assessment of N-MYC gene status in FFPE tissue (neuroblastoma patient tissue).

Information

Name

Catalogue number

Packing size

Prize

Information for use

LSI N-MYC (Orange)

IM_020

200μl

upon request

pdf

References

  • Black CT, Andrassy RJ, McNeese AG: Neuroblastoma in Pediatric Surgical Oncology, pages 175-213 . The Curtis Center, Philadelphia, USA, 1998.
  • Bown N, Cotteril S, Lastowska M, O´Neill S, Pearson ADJ, Plantaz D, et al.: Gain of chromosome arm 17q and adverse outcome in patients with neuroblastoma. N Engl J Med 1999; 340: 1954-61. [fulltext]
  • Heim S., Mitelman F.: Cancer cytogenetic; Chromosomal and molecular genetic aberrations of tumor cells, A John Wiley and sons, Inc. press, New York 1995, Second edition.