LSI EGFR (Orange)

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Probe location

EGFR 

Probe description

The LSI EGFR FISH kit is intended for the determination of EGFR gene status in human tissues using fluorescence in situ hybridization (FISH).
The EGFR FISH kit contains a directly labeled fluorescent DNA probe in hybridization buffer. The fluorochrome Orange labeled EGFR probe covers the chromosome 7p12 region.
The EGFR gene codes for the epidermal growth factor receptor (HER1, erbB-1). EGFR gene plays an important role in the regulation of the cell cycle, cell proliferation, differentiation, and the epidermal tissue cell survival.  Mutations, amplifications or misregulations of EGFR gene have been associated with a number of cancers, e. g. lung cancer, colorectal cancer, breast cancer and glioblastoma.

FISH results

Normal cell (Fig.1a) shows 2 orange signals (EGFR). An abnormal cell (Fig.1b) shows higher copy number of EGFR gene.

Normal cell in FFPE tissue hybridized with LSI EGFR (Orange) shows 2 orange signals. An abnormal cell in FFPE tissue hybridized with LSI EGFR (Orange) shows a higher copy number of EGFR gene.
1a 1b

Figure. 1. Assessment of the copy number of EGFR gene in FFPE tissue (lung cancer patient tissue).
1a) 2 copies of EGFR gene in cell.
1b) Higher copy number of EGFR gene in cell (amplification).

Information

Name

Catalogue number

Packing size

Prize

Instruction for use

LSI EGFR (Orange)

IM_033

200μl

upon request

pdf

 

References

  • Smith JS, Tachibana I, Passe SM, Huntley BK, Borell TJ, Iturria N, O'Fallon JR, Schaefer PL, Scheithauer BW, James CD, Buckner JC, Jenkins RB: PTEN mutation, EGFR amplification, and outcome in patients with anaplastic astrocytoma and glioblastoma multiforme. J Natl Cancer Inst. 2001 Aug 15;93(16):1246-56. [fulltext]
  • Reade CA, Ganti AK: EGFR targeted therapy in non-small cell lung cancer: potential role of cetuximab.Biologics. 2009;3:215-24. [fulltext]
  • Gialeli C, Kletsas D, Mavroudis D, Kalofonos HP, Tzanakakis GN, Karamanos NK. Targeting Epidermal Growth Factor Receptor in Solid Tumors: Critical Evaluation of the Biological Importance of Therapeutic Monoclonal Antibodies. Curr Med Chem. 2009 Sep 13. [Epub ahead of print]. [fulltext]