LSI Cyclin D1 (Orange)/CEP11 (Green)

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Probe location

Cyclin D1

Probe description

The LSI Cyclin D1 FISH kit is intended for the determination of Cyclin D1 gene amplification in human tissues using fluorescence in situ hybridization (FISH).
The Cyclin D1 FISH kit contains two directly labeled fluorescent DNA probes in hybridization buffer. The fluorochrome Orange labeled Cyclin D1 probe covers the chromosome 11q13 region. The fluorochrome Green labeled chromosome enumeration probe CEP11 covers the chromosome 11p11.1-q11.1 region.
The protein encoded by Cyclin D1 (CCND1) gene belongs to the highly conserved cyclin family. Cyclins and cyclin-dependent kinases (CDK) represent  the main regulatory systems of the cell cycle. The synthesis of Cyclin D1 is initiated during G1 and drives through the G1/S phase transition. Gene amplification or an increased production of the Cyclin D1 protein can be found in the different types of cancer, e. g. primary breast carcinoma, ovarian carcinoma, hepatocellular carcinoma, and small-cell lung carcinoma.

FISH results

A normal cell in FFPE tissue hybridized with LSI Cyclin D1 (Orange)/CEP11 (Green) shows 2 orange signals of Cyclin D1 gene and 2 green signals of chromosome 11. An abnormal cell in FFPE tissue hybridized with LSI Cyclin D1 (Orange)/CEP11 (Green) shows a higher copy number of Cyclin D1 gene and 2 green signals of chromosome 11.
A B


Figure. 1: The assessment of Cyclin D1 gene copy number and copy number of chromosome 11 in the FFPE tissue.         
red LSI Cyclin D1 
green CEP11
a) 2 copies of Cyclin D1 gene and 2 copies of chromosome 11 in a normal cell (HL60S cell line).
b) 2 copies of chromosome 11 and higher copy number of Cyclin D1 gene (amplification) in an abnormal cell (SKMEL2 cell line).

Information

Name

Catalogue number

Packing size

Prize

Information for use

LSI Cyclin D1 (Orange)/CEP11 (Green)

IM_030

200μl

upon request

pdf

References

  • Bates S, Peters G: Cyclin D1 as a cellular proto-oncogene. Semin Cancer Biol 1995; 6: 73-82. [fulltext]
  • Courjal F, Louason G, Speiser P, Katsaros D, Zeillinger R, Theillet C: Cyclin gene amplification and overexpression in breast cancer and ovarian cancers: Evidence for the selection of cyclin D1 in breast and cyclin E in ovarian tumors. Int J Cancer 1996; 69: 247-253. [fulltext]
  • Imoto M, Doki Y, Jiang W, Kyu-Ho E, Weinstein IB: Effects of Cyclin D1 overexpression on G1 progression – related events. Exp Cell Res 1997; 236: 173-180.Sherr CI: Cancer cell cycles. Science 1996; 274: 1672-77. [fulltext]
  • Simpson JF, Quan DE, O´Malley F, Odom-Maryon T, Clarke PE: Amplification of CCND1 and expression of its protein product, cyclin D1, in ductal carcinoma in situ of the breast. Am J Pathol 1997; 151(1): 161-168. [fulltext]
  • Terada Y, Okado T, Inoshita S, Hanada S, Kuwahara M, Sasaki S, et al.: Glucocorticoids stimulate p21CIP1 in mesengial cells and in anti-GMB glomerulonephritis. Kidney Int 2001; 59: 1706-16. [fulltext]