LSI C-MYC (Orange)/CEP8 (Green)

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C-MYC

Probe description

The LSI C-MYC FISH kit is intended for the determination of C-MYC gene status in human tissues using fluorescence in situ hybridization (FISH).
The C-MYC FISH kit contains two directly labeled fluorescent DNA probes in hybridization buffer. The fluorochrome Orange labeled C-MYC probe covers the chromosome 8q24 region. The fluorochrome Green labeled chromosome enumeration CEP8 probe covers the chromosome 8p11.1-q11.1 region.
The C-MYC gene (human homolog of v-MYC gene isolated from the avian myelocytomatosis virus) encodes a eukaryotic DNA-binding protein that activates expression of many genes. C-MYC is activated in most tumors. In addition, several tumors contain genetic alterations (i.e. translocations, gene amplifications and mutations in C-MYC regulators), which directly affect C-MYC expression and/or activity.

FISH results

A normal cell in FFPE tissue hybridized with LSI C-MYC (Orange)/CEP8 (Green) shows 2 orange signals of C-MYC gene and 2 green signals of chromosome 8. An abnormal cell in FFPE tissue hybridized with LSI C-MYC (Orange)/CEP8 (Green) shows a higher copy number of C-MYC gene and 2 green signals of chromosome 8.
A B

Figure. 1: The assessment of C-MYC copy number and copy number of chromosome 8 in FFPE tissue.         
red LSI C-MYC 
green CEP8
a) 2 copies of C-MYC gene and 2 copies of chromosome 8 in normal cell (HL60S cell line).
b) 2 copies of chromosome 8 and higher copy number of C-MYC gene in an abnormal cell (cell line NCiN417) .

Information

Name

Catalogue number 

Packing size

Prize

Information for use

LSI C-MYC (Orange)/CEP8 (Green)

IM_022

200μl

upon request

 

References

  • Spencer CA, Groudine M. Control of c-myc regulation in normal and neoplastic cells. Adv Cancer Res. 1991;56:1-48.
  • Nesbit CE, Tersak JM, Prochownik EV. MYC oncogenes and human neoplastic disease. Oncogene. 1999 May 13;18(19):3004-16.