LSI 9p21.3 (Orange)

Probe location

9p21.3

Probe description

The LSI 9p21.3 FISH kit is intended for the determination of the 9p21.3 deletion in human tissues using fluorescence in situ hybridization (FISH).
The LSI 9p21.3 FISH kit contains a directly labeled fluorescent DNA probe in hybridization buffer. The fluorochrome Orange labeled probe covers the chromosome 9p21.3 region.
The tumor suppressor gene CDKN2A (Cyclin-Dependent Kinase Inhibitor 2A) encoding protein p16, which regulates the cell cycle, is localized in the chromosomal region 9p21.3. The short arm of the chromosome 9 is frequently deleted in human cancers and correlated with an unfavorable prognosis. The use of the 9p21.3 probe with other relevant markers (e.g. 1p36.3, 19q13, and 10p11.1) is important in the complex genetic diagnostics of malignant gliomas.

FISH results

A normal cell hybridized with the LSI 9p21.3 (Orange) (1a). The cell in this image shows 2 orange signals. An abnormal cell (1b) shows the one orange signal which indicates the 9p21.3 deletion.


1a	1b

Fugure 1. The assessment of 9p21.3 status in FFPE tissue (glioma patient tissue).

Information

Name	Catalogue number	Packing size	Prize	Information for use
LSI 9p21.3 (Orange)	IM_004	200μl	upon request	pdf

Reference

Shete S, Hosking FJ, Robertson LB, Dobbins SE, Sanson M, Malmer B, Simon M, Marie Y, Boisselier B, Delattre JY, Hoang-Xuan K, El Hallani S, Idbaih A, Zelenika D, Andersson U, Henriksson R, Bergenheim AT, Feychting M, Lönn S, Ahlbom A, Schramm J, Linnebank M, Hemminki K, Kumar R, Hepworth SJ, Price A, Armstrong G, Liu Y, Gu X, Yu R, Lau C, Schoemaker M, Muir K, Swerdlow A, Lathrop M, Bondy M, Houlston RS. Genome-wide association study identifies five susceptibility loci for glioma. Nat Genet. 2009 Aug;41(8):899-904.