LSI 19q13 (Orange)

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Probe location

19q13

Probe description

The LSI 19q13 FISH kit is intended for the determination of the 19q13 chromosomal region deletion in human tissues using fluorescence in situ hybridization (FISH).
The LSI 19q13 FISH kit contains a directly labeled fluorescent DNA probe in hybridization buffer. The fluorochrome Orange labeled probe 19q13 covers the chromosome 19q13 region.
The 19q13 deletion together with the deletion of short arm of chromosome 1 (1p36.3) are strongly correlated with gliomas with oligodendroglial morphology. Combined deletions of 1p and 19q are found in approximately 80–90 % of grade II oligodendrogliomas and in approximately 50–70 % of anaplastic oligodendrogliomas.  Analysis of the 19q13 deletion is useful for morphological and genetic diagnostics of malignant tumors with an oligodendroglial component.

FISH results

A normal cell hybridized with the LSI 19q13 (Orange) (1a). The cell in this image shows 2 orange signals. An abnormal cell (1b) shows the one orange signal which indicates the 19q13 deletion.

A normal cell hybridized with the LSI 19q13 (Orange) shows 2 orange signals of chromosome 19q13 region. An abnormal cell hybridized with the LSI 19q13 (Orange) shows the one orange signal which indicates the loss of chromosome 19q13 region.
1a 1b

Figure1. The assessment of 19q13 status in FFPE tissue (glioma patient tissue).

Information

Name

Catalogue number

Packing size

Prize

Information for use

LSI 19q13 (Orange)

IM_006

200μl

upon request

pdf

References

  • Guppy KH, Akins PT, Moes GS, Prados MD. Spinal cord oligodendroglioma with 1p and 19q deletions presenting with cerebral oligodendrogliomatosis. J Neurosurg Spine. 2009 Jun;10(6):557-63. [fulltext]
  • Weller M, Felsberg J, Hartmann C, Berger H, Steinbach JP, Schramm J, Westphal M, Schackert G, Simon M, Tonn JC, Heese O, Krex D, Nikkhah G, Pietsch T, Wiestler O, Reifenberger G, von Deimling A, Loeffler M. Molecular Predictors of Progression-Free and Overall Survival in Patients With Newly Diagnosed Glioblastoma: A Prospective Translational Study of the German Glioma Network. J Clin Oncol. 2009 Oct 5. [Epub ahead of print] [fulltext]
  • Gadji M, Fortin D, Tsanaclis AM, Drouin R. Is the 1p/19q deletion a diagnostic marker of oligodendrogliomas? Cancer Genet Cytogenet. 2009 Oct;194(1):12-22. [fulltext]